Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL
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Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL
1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.
Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL
Numéro d'article: 51556604

Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

Numéro d'article: 51556604

MGA 215492

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1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.
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Ce qui se démarque

Sterile Solution
Ensures contamination-free experiments, enhancing the reliability and accuracy of your research outcomes with ready-to-use phosphate buffered saline.
Convenient Volume
500 mL quantity provides ample supply for multiple applications, reducing the need for frequent reorders and saving laboratory time and resources.
Optimal pH Stability
Maintains a stable pH environment, crucial for biological experiments, ensuring consistent performance across various applications in cell culture and immunology.

Détails du produit

Get high-quality 1X PBS for cell culture applications. Sterile, filter-sterilized solution with balanced, isotonic salt composition. pH 7.4. Research use only. Shop at Ubuy Madagascar
  • Manufactured in USA in ISO 9001 and 13485 certified facility
  • For research use only, not for human or animal clinical/therapeutic use
  • Each production lot undergoes stringent Good Manufacturing Practices and quality control tests
  • 1X Phosphate Buffered Saline (PBS) is 0.22 micron filter-sterilized
  • Contains 137mM NaCl, 10mM NaH2PO4, 2.7mM KCl, 1.8mM KH2PO4 with pH 7.4
  • Used for cell culture applications such as washing cells, transporting cells, and preparing reagents
Item Weight1.1 lbs (500 grams)

À qui est-ce destiné ?

Suitable For
  • Laboratory Research

    Ideal for researchers conducting cell culture, protein purification, and immunoassays requiring a stable pH environment.

  • Diagnostic Testing

    Useful for labs performing diagnostic tests, providing a sterile and buffered solution for sample preparation.

  • Biological Experiments

    Suitable for biological experiments where maintaining osmotic balance and pH stability is critical for results.

Not Suitable For
  • Long-term Storage

    Not suitable for long-term storage of cells or samples due to potential degradation and nutrient depletion.

  • Non-scientific Use

    Not recommended for any applications outside of laboratory settings or scientific research and experimentation.

  • High Concentration Needs

    May not be suitable for high-concentration scenarios where concentrated solutions are required for specific applications.

DESCRIPTION DU PRODUIT

Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL

Vous avez une question ? Chattez avec nous

Questions et réponses des clients

  • question: What is Phosphate Buffered Saline (PBS) used for?

    répondre: Phosphate Buffered Saline (PBS) is primarily used in biological research as a buffer solution for dissolving biological samples. Its formulation helps maintain a stable pH, making it ideal for applications like cell culture, immunoassays, and molecular biology experiments. The sterile nature of PBS prevents contamination, ensuring accurate results. For example, in cell culture, PBS is frequently used to rinse cells before trypsinization, as it helps maintain osmotic balance and cell viability.
  • question: Is PBS 1X suitable for use in cell culture?

    répondre: Yes, PBS 1X is suitable for cell culture applications. It provides a balanced salt solution that supports the physiological conditions necessary for cell survival and growth. With its sterile formulation, PBS helps in processes such as washing cells, preparing samples for flow cytometry, and diluting other reagents. Many researchers use PBS to remove serum before enzymatic detachment of adherent cells, ensuring minimal disruption to experimental results.
  • question: How should Phosphate Buffered Saline be stored?

    répondre: Phosphate Buffered Saline should be stored at room temperature in a cool, dry place, away from direct sunlight. It is recommended to keep PBS tightly sealed in its original container to prevent contamination. While PBS can be stored for extended periods, it is advisable to check for any visible contaminants before use. Proper storage ensures the buffer retains its efficacy, which is critical for sensitive applications such as immunofluorescence staining.
  • question: Can PBS be used in molecular biology techniques?

    répondre: Yes, PBS is widely used in various molecular biology techniques, including PCR (Polymerase Chain Reaction), Western blotting, and ELISA (Enzyme-linked Immunosorbent Assay). Its role as a buffering agent aids in maintaining a stable pH during reactions, which is crucial for enzyme activity and specificity. For instance, during the dilution of antibodies or nucleic acids, PBS helps maintain the integrity of the reagents, contributing to reproducible experimental outcomes.
  • question: Is PBS 1X compatible with mammalian cells?

    répondre: PBS 1X is compatible with mammalian cells, as it mimics physiological conditions, making it an ideal medium for cell handling and experiments. Its isotonic nature prevents osmotic shock, ensuring cell health during procedures like trypsinization or washing. Researchers often utilize PBS when preparing mammalian cells for flow cytometry assays, as it aids in maintaining cell viability while optimizing staining protocols.
  • question: What are the components of Phosphate Buffered Saline?

    répondre: Phosphate Buffered Saline typically contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. These components work together to create an isotonic and buffered environment, maintaining a stable pH around 7.4. This balanced formulation is crucial for biological applications, as it prevents changes in cellular environments that could disrupt normal function. Understanding these components can help researchers select the right medium for their specific experiments.
  • question: Can I use PBS for preparing samples for flow cytometry?

    répondre: Absolutely, PBS is a preferred buffer for preparing samples for flow cytometry. Its isotonic properties help preserve cell morphology and viability, which are essential for accurate analysis. By using PBS to dilute samples, researchers can effectively minimize background noise and improve the clarity of cytometric data. Additionally, many protocols recommend washing cells with PBS to reduce nonspecific binding of antibodies, enhancing the quality of results.
  • question: Is the PBS 1X solution sterile?

    répondre: Yes, PBS 1X is available in a sterile formulation, ensuring it is free from microbial contamination. This sterility is critical in laboratory settings where contamination can compromise experimental integrity. For example, using sterile PBS is essential when handling cultured cells or preparing immunological assays to prevent bacterial or fungal interference. Always check that the packaging is intact to maintain the sterility of the solution before use.
  • question: Can I use PBS for washing tissue samples?

    répondre: Yes, PBS is commonly used for washing tissue samples before further processing or analysis. Its saline composition helps maintain osmotic balance, preventing tissue distortion during washing. This is particularly important when preparing samples for histological examination or when conducting assays that require intact tissue morphology. Many researchers utilize PBS to wash samples thoroughly, ensuring accurate results in subsequent steps.
  • question: Where can I buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL?

    répondre: You can buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL at Ubuy in Madagascar. Ubuy offers a wide selection of laboratory supplies, ensuring availability and convenience for researchers looking for high-quality PBS solutions. Shopping through Ubuy gives you access to reliable products tailored for scientific applications.

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  • Limitations : Pour les produits expédiés à l'international, veuillez noter que toute garantie du fabricant peut ne pas être valide ; les options de service du fabricant peuvent ne pas être disponibles ; les manuels, instructions et avertissements de sécurité des produits peuvent ne pas être dans les langues du pays de destination ; les produits (et les matériaux qui les accompagnent) peuvent ne pas être conçus conformément aux normes, spécifications et exigences d'étiquetage du pays de destination ; et les produits peuvent ne pas être conformes à la tension et aux autres normes électriques du pays de destination (nécessitant l'utilisation d'un adaptateur ou d'un convertisseur le cas échéant). Il incombe au destinataire de s'assurer que le produit peut être importé légalement dans le pays de destination. En cas de commande auprès d'Ubuy ou de ses filiales, le destinataire est l'importateur officiel et doit se conformer à toutes les lois et réglementations du pays de destination.
  • Tous les produits listés sur Ubuy ne sont pas à vendre, Ubuy étant un moteur de recherche mondial. Les produits sont soumis aux réglementations en matière d'exportation et de commerce.

MGA 215492

Commandez maintenant et recevez votre commande aux alentours du Mercredi, Juillet 15

QTY:

Transaction sécuriséeTransaction sécurisée

Ubuy s'engage à protéger votre sécurité et votre confidentialité. Notre système avancé de sécurité des paiements garantit la confidentialité en chiffrant vos informations lors de la transmission grâce aux protocoles AES (Advanced Encryption Standards) et SSL (Secure Socket Layer). Vos coordonnées de paiement sont 100 % sécurisées car nous ne partageons pas vos informations de paiement avec des vendeurs tiers.

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Caractéristiques et avantages

  • Contains a balanced, isotonic salt solution
  • Used to wash cells before dissociation
  • Transporting cells or tissue
  • Diluting cells for counting
  • Preparing numerous reagents
  • Manufactured under certified 14644 clean room conditions in USA lab